Journal:

Article Title: GLO1 Overexpression in Human Malignant Melanoma

doi: 10.1097/CMR.0b013e3283364903

Figure Lengend Snippet: (A) 44 Human malignant melanoma tissue specimens on a TNM-staged melanoma TMA (IMH-369, IMGENEX HISTO-ArrayTM, depicted in panel C, left portion) containing 44 human malignant melanoma tissue specimens and 7 healthy control skin samples (control, IMH-323) were analyzed by immunohistochemical staining for glyoxalase 1. Average histological (H) scores were calculated by multiplying intensity by prevalence. (B) H-score analysis depicted as dot blot. Comparison between groups [control (n=7); stage II-III (n=33); stage IV (n=11)] was done using the two-sided Student’s t-test (*p<0.05; **p<0.01; ***p<0.001; lines indicate average values). (C) Melanoma-TMA IMH-369 (left) with tissue samples that represent the range of H-scores: (from left to right) non-melanoma healthy control (H-score: 0), melanoma samples (H-scores: I-III).

Article Snippet: Using a human melanoma tissue array containing first strand cDNAs (Human Melanoma TissueScan Real-Time Panel I, MERT101, ORIGENE, Rockville, MD) prepared from 40 human melanoma tissues (stages III and IV) and 13 healthy control skin samples (HMRT102, Origene), mRNA tissue levels (GLO1 versus ACTB) were determined by quantitative RT-PCR analysis following the manufacturer’s instructions using an Applied Biosystems 7000 SDS and Applied Biosystems’ Assays On Demand primers specific to GLO1 (assay ID Hs00198702_m1) and ACTB (β-actin, assay ID Hs99999903_m1).

Techniques: Control, Immunohistochemical staining, Staining, Dot Blot, Comparison

Journal:

Article Title: GLO1 Overexpression in Human Malignant Melanoma

doi: 10.1097/CMR.0b013e3283364903

Figure Lengend Snippet: (A) Using a human melanoma tissue-scan real-time panel containing first strand cDNAs prepared from 40 human melanoma tissues (stages III and IV) and 13 healthy controls, mRNA tissue levels (GLO1 versus ACTB) were determined by quantitative RT-PCR analysis. Every tissue sample was analyzed in duplicate. (B) Dot blot depiction of GLO1 mRNA expression levels. Comparison between groups [control (n=13); stage III (n=21); stage IV (n=19)] was done using the two-sided Student’s t-test (*p<0.05; **p<0.01; ***p<0.001; lines indicate average values).

Article Snippet: Using a human melanoma tissue array containing first strand cDNAs (Human Melanoma TissueScan Real-Time Panel I, MERT101, ORIGENE, Rockville, MD) prepared from 40 human melanoma tissues (stages III and IV) and 13 healthy control skin samples (HMRT102, Origene), mRNA tissue levels (GLO1 versus ACTB) were determined by quantitative RT-PCR analysis following the manufacturer’s instructions using an Applied Biosystems 7000 SDS and Applied Biosystems’ Assays On Demand primers specific to GLO1 (assay ID Hs00198702_m1) and ACTB (β-actin, assay ID Hs99999903_m1).

Techniques: Quantitative RT-PCR, Dot Blot, Expressing, Comparison, Control